National survey of tuberculosis prevalence in Viet Nam
Nguyen Binh Hoa a, Dinh Ngoc Sy a, Nguyen Viet Nhung a, Edine W Tiemersma b, Martien W Borgdorff c & Frank GJ Cobelens c
a. National Tuberculosis Programme Viet Nam, Hanoi, Viet Nam.
b. KNCV Tuberculosis Foundation, PO Box 146, 2501 CC The Hague, Netherlands.
c. Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.
C. orrespondence to Edine W Tiemersma (e-mail: email@example.com).
(Submitted: 28 May 2009 – Revised version received: 18 September 2009 – Accepted: 20 September 2009 – Published online: 22 February 2010.)
Bulletin of the World Health Organization 2010;88:273-280. doi: 10.2471/BLT.09.067801
Tuberculosis is still a major cause of morbidity and mortality worldwide. In 2006, an estimated 9.2 million new tuberculosis cases occurred, resulting in 1.7 million deaths.1 The Viet Nam National Tuberculosis Control Programme (NTP) is based on the principles of DOTS, the core control strategy recommended by the World Health Organization (WHO).2 According to WHO estimates, since 1997 Viet Nam has reached and exceeded the global targets for tuberculosis control,2,3 i.e. to detect > 70% of new smear-positive pulmonary tuberculosis cases and cure > 85% of these detected cases.1 If these targets were met, tuberculosis incidence in Viet Nam would predictably decrease over the period 1997–2004 by 44%.3,4 Although there was indeed a small decrease in tuberculosis notification rates among women and persons older than 35 years, this was offset by an increase among young men, which led to stabilization in notification rates during this period.3 In 2006, WHO estimated that Viet Nam ranked 12th among the tuberculosis high-burden countries1, with a prevalence of smear-positive tuberculosis of 89 per 100 000 population. (WHO, unpublished data, 2008).
A major question for the Viet Nam NTP is whether these estimates are correct. However, the tuberculosis burden and case detection rate are unknown, because they can only be measured directly if the incidence is known. Tuberculosis incidence is difficult to measure, but tuberculosis prevalence can be assessed by cross-sectional surveys and can be used to estimate the rate at which tuberculosis patients are detected and put on treatment as part of a tuberculosis control programme (i.e. the patient diagnostic rate, PDR).5,6 The PDR provides information about the burden of tuberculosis and, if determined at different times, can show the impact of tuberculosis control measures. We aimed to measure the burden of tuberculosis disease, as revealed by a nationwide representative cross-sectional survey, by estimating the tuberculosis prevalence in Viet Nam and comparing this to the tuberculosis prevalence estimated by WHO1. We also identified the major demographic determinants of tuberculosis prevalence.
Study setting and population
We did a population-based cross-sectional survey based on multistage cluster sampling, stratified by urban, rural and remote areas. A sample size of approximately 105 000 adults (15 years or older) was calculated based on 95% confidence interval (CI) boundaries of 71 and 129 per 100 000 for an observed prevalence of 100 per 100 000 population1 and a design effect factor due to cluster sampling of 1.5.7
Clusters had an approximate population size of 2240 inhabitants (1500 adults), and 70 clusters were selected. Districts were selected with sampling probability proportional to the population in the 1999 census (General Statistics Office of Vietnam, unpublished data, 2002). In the selected districts, communes and sub-communes were selected by simple random sampling. Briefly, all communes or sub-communes were assigned a random number and in each district one was selected and used as the starting point. Other sub-communes were added until the required number of inhabitants (2240 people) was obtained by first going north from the selected sub-commune and then adding sub-communes along a clockwise route.
Smear-positive tuberculosis is usually rare in people younger than 15 years, and it is difficult to collect sputum samples from children. Therefore the population eligible for the survey included all residents aged ≥ 15 years in the selected clusters who were present during the survey census. A resident was defined as a person who had lived in the household concerned for at least 3 months. To avoid participation bias, short-term guests in households were excluded from the survey.
The survey was conducted from September 2006 to July 2007. In each cluster, data were collected during 14-day periods. First, the survey team conducted a house-to-house census based on population lists provided by the local authorities, to see who was present, to inform survey subjects about procedures and to collect household data. Subsequently persons suspected of having tuberculosis were identified by screening all eligible inhabitants during an interview and by chest X-ray with mobile X-ray units. Individuals with findings suggestive of tuberculosis were interviewed in depth and were asked to submit three sputum specimens. The first and third specimens were examined by smear test by the survey team in the district laboratory. The second (morning) specimen was transported to national and regional reference laboratories for smear examination, culture and species identification. At the reference laboratories, all slides were stored for selection of all (100%) positive smears and a 10% random sample of all negative smears from each sampling unit was used for re-reading. All X-ray images were transported to central X-ray reading units for re-reading of 100% of those scored as abnormal, a 20% random sample of those scored as normal in the field in the first 20 clusters and a 10% random sample of those scored normal in the subsequent 50 clusters. For this purpose, all X-ray images were given a serial number in chronological order, and a serial number of < 10 was="" randomly="" chosen.="" then="" every="" tenth="" subsequent="" serial="" number="" was="" chosen="" for="" re-reading.="" for="" example,="" if="" serial="" number 7="" was="" chosen,="" the="" 17th,="" 27th,="" etc.="" images="" were=""> 10>
A person suspected of having tuberculosis was defined as any survey participant with chest X-ray abnormalities consistent with tuberculosis, productive cough for more than 2 weeks, current tuberculosis treatment or a history of tuberculosis in the preceding 2 years. A smear-positive pulmonary tuberculosis case was defined by the presence of either two or more positive smears, one positive smear plus an abnormal chest X-ray result consistent with tuberculosis, or one positive smear plus a positive culture. A culture-positive case was defined as a participant with a positive culture. A bacteriologically-confirmed case was defined as a case with smear-positive or culture-positive pulmonary tuberculosis (or both). A new case was defined as a participant who had never had treatment for tuberculosis or who had taken anti-tuberculosis drugs for <>8,9 Final classification of each tuberculosis case was done by a consensus panel that included an international expert not involved in the design or implementation of the study.
All data were double-entered in an EpiData Software database (EpiData Association, Odense, Denmark); discrepancies were checked against the raw data. Data were analysed with Stata version 9SE software (Stata Corporation, College Station, TX, United States of America). Our primary analysis calculated overall prevalence rates; additional subgroup analyses were done to estimate stratum-specific prevalence rates by age group, sex and geographic area. In all analyses, population weightings were used to adjust for the stratified sampling design, for differences between clusters in population growth since 1999 and for differences in cluster size. Differences in departure from the cluster sample size of 1500 were found by calculating the overall exact sampling probability as:
A × B × Cwhere A is the individual sampling probability, calculated as 1 divided by the sampling interval of a given stratum. For example, the sampling interval for urban areas was 746 890; i.e. after ranking districts in decreasing number of inhabitants (largest district first), the district inhabited by every 746 891st person was selected. B is the correction factor for the differential growth in population size between the years 1999 and 2006, calculated by dividing the population of each sampled district in 2006 by the census population of that district in 1999. The correction factor C for differential cluster size was calculated by the number of participants in each cluster divided by the mean cluster size.
Point prevalence estimates, 95% CIs and design effects were calculated with the Stata “svy” commands. CIs were adjusted for the cluster design by first-order Taylor linearization.10 P-values were adjusted by the second-order correction of Rao & Scott for the Pearson χ2 test.11 To test for trends, we used the non-parametric Cuzick test.
The PDR was calculated as the number of newly-reported cases of smear-positive tuberculosis per 100 000 population per year (notification rate) divided by the prevalence of new cases of smear-positive tuberculosis per 100 000.5
The Research Board of the National Hospital for Tuberculosis and Respiratory Diseases in Hanoi gave scientific and ethical approval for the study.
The census recorded 114 389 adult residents in the selected population clusters. Of these, 103 924 (91.0%) were present on the day of the census and thus considered eligible to participate in the survey, and 94 179 (82.3%) participated (Fig. 1). With respect to age and sex, the eligible population closely reflected the demographic characteristics of the Vietnamese population; in contrast, non-participants were younger and more often male (Fig. 2).
Fig. 1. Schematic diagram of the study design and data collection in a tuberculosis prevalence survey, Viet Nam, 2006–2007
Fig. 2. Age and sex distribution of the participating population in prevalence survey versus the general population of Viet Nam, 2006–2007
Of the participants, 6444 (6.8%) had been interviewed to screen for cough, 328 (0.3%) had the results of a chest X-ray examination and 87 314 (92.7%) had both. Of the remaining 93 participants, 81 (0.10%) were screened with a chest X-ray but the results were not recorded and 12 (0.01%) were identified as suspected of having tuberculosis based on history only. Of the 87 314 participants who had both the screening interview and chest X-ray examination, 2972 (3.4%) participants suspected of having tuberculosis were identified based on chest X-ray abnormalities only, 3522 (4.0%) were identified based on productive cough only and 518 (0.6%) were identified based on both chest X-ray abnormalities and productive cough (Table 1).
Table 1. Data for productive cough and chest X-ray results, by subgroups, among 87 314 participants in a tuberculosis prevalence survey in Viet Nam, 2006–2007
The proportion of persons suspected of having tuberculosis who had both productive cough and chest X-ray abnormalities was higher among men (0.9%) than among women (0.3%). This proportion also increased with age, from 0.1% in the group aged 15–24 years to 2.2% in the group aged ≥ 65 years. Chest X-ray abnormalities, with or without productive cough, were more frequent in the southern (4.6%) than in the northern (3.7%) or the central (3.6%) region of the country, but the differences were not statistically significant.
Smear examination, culture or both tests done for 7648 participants identified 269 tuberculosis cases, and 174 of these were smear-positive (Fig. 1). Six participants (3.4%) were on tuberculosis treatment, 16 (9.2%) had a history of tuberculosis treatment in the preceding 2 years and three (1.7%) had both current and past tuberculosis treatment. Fourteen participants (8.0%) had received tuberculosis treatment more than 2 years before the survey.
In-depth interview data were available for 264 participants with tuberculosis (98.1%). Productive cough for more than 2 weeks was reported by 131 (49.6%), any cough for more than 2 weeks by 145 (54.9%), any cough of any duration by 178 (67.4%) and any symptom suggestive of tuberculosis by 196 (74.2%). There were no significant differences in the frequency of cough or other symptoms between smear-positive and smear-negative cases (data not shown).
Based on 174 identified cases among 94 179 participants, the weighted prevalence of smear-positive tuberculosis was 196.8 per 100 000. The prevalence rate of smear-positive tuberculosis was higher in men than in women (351.1 versus 69.3 per 100 000; P < 0.001)="" and="" increased="" significantly="" with="" age,="" from="" 42.2="" per="" 100 000="" in="" the="" group="" aged="" 15–24="" years="" to="" 429.3="" per="" 100 000="" in="" the="" group="" aged="" ≥ 65="">
Table 2. Estimated prevalence of tuberculosis in Viet Nam, according to sputum smear-positive and bacteriologically confirmed cases in survey participants ≥ 15 years old, 2006–2007
The prevalence of smear-positive tuberculosis was significantly lower in remote areas (134.3 per 100 000) as compared with urban (203.2 per 100 000) and rural areas (219.4 per 100 000). It was also higher in southern than in central and northern Viet Nam; this difference approached statistical significance (Table 2).
The weighted prevalence of bacteriologically-positive tuberculosis was 307.2 per 100 000 adult population (95% CI: 248.8–365.6) (Table 2). The prevalence rate of bacteriologically-positive tuberculosis was also significantly higher in men than in women (536.4 versus 117.8 per 100 000; P < 0.001).="" as="" with="" smear-positive="" tuberculosis,="" the="" prevalence="" rate="" of="" bacteriologically="" confirmed="" disease="" was="" lower="" in="" remote="" (232.3="" per="" 100 000)="" than="" in="" urban="" (282.0="" per="" 100 000)="" and="" rural="" areas="" (344.2="" per="" 100 000),="" but="" this="" difference="" was="" not="" significant.="" in="" addition,="" it="" was="" lower="" in="" central="" (209.4="" per="" 100 000)="" than="" in="" southern="" (367.4="" per="" 100 000)="" or="" northern="" viet="" nam="" (286.1="" per="">
A detailed analysis of the age-specific and sex-specific prevalence rates showed male-to-female ratios of 1.9 or higher, irrespective of how a tuberculosis case was defined. A similar pattern was observed for chest X-ray abnormalities consistent with tuberculosis. Overall, the prevalence rate of smear-positive tuberculosis in men was 5.1 times as high as in women, and the prevalence rate of all bacteriologically confirmed tuberculosis was 4.6 times as high in men as in women (Table 3).
Table 3. Male-to-female ratios of the tuberculosis prevalence ratea per 100 000 persons ≥ 15 years, by age group, in Viet Nam, 2006–2007
The PDR of new smear-positive tuberculosis in the year 2006 was 0.60 (95% CI: 0.49–0.78). This PDR was higher among women (0.86) than among men (0.50), highest in the group aged 25–34 years (0.94) and lowest in the group aged 35–44 years (0.56). The PDR was slightly higher in the southern (0.66) than in the northern and central regions of Viet Nam (0.54), as shown in Table 4.
Table 4. Estimated patient diagnosis rates for the Viet Nam National Tuberculosis Programme, by sex, age group and geographic region, 2006–2007
The survey showed a weighted prevalence rate of smear-positive tuberculosis among the population aged ≥ 15 years of 196.8 per 100 000. Assuming that there were no smear-positive tuberculosis cases among the population younger than 15 years, the national prevalence rate of smear-positive tuberculosis in the total population (all age groups) would be 145 per 100 000 (95% CI: 110–180). This number is 1.6 times as high as the previous WHO estimate for Viet Nam in 2006 (89 per 100 000). Thus, the burden of tuberculosis in Viet Nam is probably considerably greater than assumed. Although we did not measure morbidity or mortality in this survey, this prevalence represents substantial morbidity and mortality directly related to tuberculosis and, potentially, to other diseases.12 WHO estimates, based on tuberculin survey data from the 1990s,13 are clearly too low, and the difference between the earlier estimate and our figures illustrates that tuberculosis prevalence, and probably incidence, cannot be reliably inferred from such data with Styblo’s rule.14,15
Of the 174 smear-positive cases detected, 39 (22%) were currently on tuberculosis treatment or had been treated for tuberculosis for ≥ 1 month. Thus, most smear-positive cases were not known to the NTP.
Tuberculosis control in Viet Nam is based on passive case-finding among patients who present with persistent productive cough.2 In this survey, only 53% of those identified with smear-positive tuberculosis indicated they had persistent productive cough. Thus, a considerable proportion of the tuberculosis cases we detected would not have been diagnosed in NTP facilities although some of the cases (specifically those with mild complaints) would probably have been diagnosed by the NTP at a later time.
Smear-positive cases accounted for 64% of all bacteriologically-positive cases. This proportion was relatively high compared to other recent prevalence surveys within the region16–19 and may reflect the dynamics of the tuberculosis epidemic in Viet Nam. Our higher figure may also be explained by the fact that only the second sputum specimen was cultured, a procedure that may be less sensitive than culturing two specimens, as was done in the other surveys. We may therefore have underestimated the prevalence of culture-positive tuberculosis.
Most smear-positive tuberculosis cases (89%) had chest X-ray abnormalities consistent with tuberculosis. This association suggests that, together with direct smear examination, chest X-ray may be an important tool for tuberculosis case-finding. There was a strong and statistically significant correlation with age, both for the prevalence of chest X-ray abnormalities and persistent productive cough, and for the prevalence of bacteriologically-confirmed tuberculosis. Tuberculosis notification rates in Viet Nam also tend to increase with age.
Interestingly, the increase in prevalence rate occurred at a much earlier age in men than in women, which was reflected in very high male-to-female ratios in the group aged 45–64 years. The overall male-to-female ratios for the prevalence rate of tuberculosis were 4.5 to 5.1, depending on the type of tuberculosis (Table 3). This is 1.7 times as high as the notification rate of new smear-positive tuberculosis (2.8) in the NTP in 2006. Our higher ratio strongly suggests that the sex difference in tuberculosis notification in Viet Nam reflects a true difference in disease occurrence rather than a difference in access to diagnosis and treatment, as some studies have suggested.16,20–22 A nationwide study carried out in 2002 in the same 70 clusters we used showed longer delays in diagnosis among women than among men (although the difference was small),20 but an analysis of tuberculosis laboratory data in the north of Viet Nam suggested that women were more likely than men to have or request a sputum smear examination.23
In Viet Nam, the considerably higher PDR in women (0.86) compared with men (0.50) suggests that the case detection rate is higher in the former. Although the PDR5 is not the same as the case detection rate, comparing the PDR between populations or subpopulations can provide information about the relative completeness of case detection. The PDR was lowest in the groups aged 35–44 (0.56) and ≥ 65 years (0.66) and lower among men than among women. This result suggests that the NTP should increase efforts to find male tuberculosis patients and focus on these two age groups. Because the PDR relates to cases found and treated by the NTP only, this raises the question of whether men, especially in the group aged 35–44 years, are diagnosed more often by private physicians than by the publicly-operated NTP.
Our survey confirmed that other characteristics of the tuberculosis notification pattern reflect features of the underlying tuberculosis epidemic in Viet Nam. Specifically, our data reflect the north–south gradient2 and the relatively low prevalence rates in remote, mountainous areas compared to the urban and low-lying rural areas.6
Although prevalence surveys such as ours are useful to evaluate the performance of tuberculosis programmes, they are logistically difficult to perform, time-consuming and expensive. Practical preparations for our survey began in mid-2005; recruitment started in September 2006 and lasted 9 months. Data entry, cleaning, analysis and reporting lasted another one-and-a-half years. Total survey costs, including the purchase of mobile X-ray units, were estimated to be 1 000 000 United States dollars.
Our survey had limitations. First, the eligible population did not include adults who were present in the sampled clusters for less than 3 months, who were incarcerated or who lived in military barracks (i.e. the mobile population) during the survey period. This is a potential drawback because it is not known how well the survey sample population represented the mobile population and thereby the total Vietnamese population. Because the mobile population probably contains a higher proportion of young men than the survey population, our survey may have either underestimated or overestimated the prevalence of tuberculosis. Young men, who were under-represented in the survey sample, tended to have a higher prevalence rate of tuberculosis than women of the same age, but a lower prevalence rate than older men. Second, rather than any cough we used persistent productive cough as a screening criterion. This might have decreased the sensitivity of our case-finding method. In fact, among the smear-positive tuberculosis cases we identified, persistent cough was more commonly mentioned in the in-depth interview (by 145 persons) than persistent productive cough (by 131 persons). Finally, this survey did not collect data on human immunodeficiency virus (HIV) infection. Further studies are needed to identify the prevalence of HIV and tuberculosis co-infection in the population and its significance for the total burden of tuberculosis in Viet Nam, because the increasing prevalence of HIV infection in parts of Viet Nam may have increased the burden of HIV and tuberculosis co-infection.24
The results of this first national prevalence survey showed a prevalence of tuberculosis in Viet Nam that was 1.6 times as high as previously estimated. A significant number of tuberculosis smear-positive and smear-negative cases remain undiagnosed and present a potential source of transmission in the community. The NTP should develop, implement and evaluate additional approaches to tuberculosis control, including special efforts to find boys and men with tuberculosis. Approaches that should be considered include: (i) the introduction of active case-finding based on chest X-ray screening in high-risk groups, and (ii) widening the eligibility criteria for smear examination to other tuberculosis-related symptoms in addition to cough. The control of tuberculosis should remain a high priority for the Vietnamese Ministry of Health.
The authors thank the Viet Nam National Tuberculosis Control Programme board of directors and tuberculosis staff involved in the survey, and all the individuals living in the study sites for taking part. They also thank Mr Nico Kalisvaart, Dr Marleen Vree and Dr Ikushi Onozaki for technical support with data management, data analysis and review of the cases of tuberculosis detected.
This study was supported financially by the Viet Nam Ministry of Health, the Government of the Netherlands, KNCV Tuberculosis Foundation, the Global Fund To Fight AIDS, Tuberculosis and Malaria, and the World Health Organization.
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