Bulletin of the World Health Organization

A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid

Lenesha Warrener, Rimantas Slibinskas, Kaw Bing Chua, Wondatir Nigatu, Kevin E Brown, Kestutis Sasnauskas, Dhanraj Samuel & David Brown

Volume 89, Number 9, September 2011, 675-682

Table 3. Results obtained for oral fluid specimens with point-of-care test (POCT) for the detection of measles-specific IgM, analysed with respect to the result quotient (T/CO) obtained in Microimmune measles-specific IgM capture enzyme immunoassay (EIA) and timing of specimen collection

EIA
No. positive by POCT out of total tested
Days between onset of symptoms and specimen collection
Total
Result T/COa 0–7 8–14 > 14 Unknown
Negative < 0.8 2/42 2/51 1/64 3/51 8/208
Equivocal 0.8 to < 1.0 0/0 0/1 0/1 0/2 0/4
Positive 1– 5 6/7 4/4 14/19 9/10 33/40
5–10 1/1 7/7 3/3 2/2 13/13
10–15 0 2/2 1/1 2/2 5/5
15–20 1/1 1/1 0 0 2/2
> 20 4/4 3/3 1/1 2/2 10/10
No. positive by POCT out of no. positive by EIA 12/13 17/17 19/24 15/16 63/70

a This represents the quotient calculated by dividing the optical density obtained for each test specimen (450/620 nm) by the cut-off value.