Laboratory biosafety manual. Second edition (revised). Interim guidelines
WHO/CDS/CSR/LYO/2003.4
Foreword
Acknowledgements
General principles
PART I Guidelines
Risk assessment
Basic laboratories – Biosafety Levels 1 and 2
The containment laboratory – Biosafety Level 3
The maximum containment laboratory – Biosafety Level 4
PART II Laboratory equipment
Biological safety cabinets
Equipment-related hazards
Equipment designed to reduce biological hazards
PART III Good microbiological technique
Safe laboratory techniques Biosafety and recombinant DNA technology
Transport of infectious substances
Contingency plans and emergency procedures
Disinfection and sterilization
PART IV Chemical, fire and electrical safety
Hazardous chemicals
Fire in the laboratory
Electrical hazards
PART V Safety organization and training
The biosafety officer and safety committee
Safety rules for support staff
Training programmes
Safety checklist
References
ANNEX 1 Immunization of staff
ANNEX 2 WHO Biosafety Collaborating Centres