Drug resistance

Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world

WHO/CDS/CSR/RMD/2003.6


Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world: Haemophilus influenzae, Neisseria meningitidis, Streptococcus pneumoniae, Neisseria gonorrhoeae, Salmonella serotype Typhi, Shigella, and Vibrio cholerae

I. Introduction
II. Requirements of a reference laboratory

Bacterial agents of pneumonia and meningitis
III. Haemophilus influenzae
IV. Neisseria meningitidis
V. Streptococcus pneumoniae

Sexually transmitted pathogen for which there are increasing antimicrobial resistance concerns
VI. Neisseria gonorrhoeae

Bacterial agents of enteric diseases of public health concern
VIII. Salmonellaserotype Typhi
VIII. Shigella
IX. Vibrio cholerae
X. Conclusion
XI. Appendices

1. Standard safety practices in the microbiology laboratory
2. Media, reagents, and quality control
3. Collection and transport of sterile site specimens
4. Isolation and presumptive identification of agents from normally sterile sites
5. Nasopharyngeal swab specimen collection and culture methodology
6. Serotyping and quellung typing of Streptococcus pneumoniae
7. Antimicrobial susceptibility testing by broth microdilution
8. Specimen collection and primary isolation of Neisseria gonorrhoeae
9. Fecal specimens:collection, transport, and field supplies
10. Laboratory processing of fecal specimens

11. Preservation and storage of isolates
12. Packing and shipping of diagnostic specimens and infectious substances
13. Manufacturer, supplier, and distributor contact information
14. International reference laboratories
15. Selected references

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