Immunization, Vaccines and Biologicals

WHO/IVB/07.07

Guidelines for Plaque-Reduction Neutralization Testing of human antibodies to Dengue viruses

The document provides guidance to do plaque-reduction neutralization (PRN) assays to assess dengue antisera. It is not a standard protocol, but a document that aims at the harmonization of assays. Based on a thorough review on the biology of the assay, it discusses its key variables and makes recommendations on how to control them. It also provides a suggested minimum protocol that is recommended to those who newly establish the assay. The protocol concludes with an outlook towards new assays, in particular those that can be automated for large sample processing.

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