WHO information for molecular diagnosis of influenza virus in humans - update
This document provides information on the following molecular diagnostic protocols available as of the above date for influenza viruses in humans.
Annex 1: Conventional RT-PCR protocols (Page 5)
- A. Conventional RT-PCR analyses for the matrix gene of influenza type A viruses (Page 5)
- B. Conventional one step RT-PCR for A(H1N1)2009 HA gene (Page 8)
- C. Conventional one step RT-PCR for A(H5N1) HA gene (Page 10)
- D. Influenza B lineage specific one step conventional RT-PCR protocol (Page 13)
- E. Conventional RT-PCR assays for the detection of influenza A(H1N1, H3N2), influenza B and influenza A(H5N1) viruses (Page 16)
Annex 2: Realtime RT-PCR protocols (Page 21)
- A. Realtime RT-PCR test for the matrix gene of influenza type A viruses (Page 21)
- B. One step realtime RT-PCR for H1 gene of A(H1N1)2009 virus (Page 23)
- C. Influenza B lineage specific one-step realtime RT-PCR (Page 26)
- D. Realtime RT-PCR assays for the detection of seasonal influenza viruses and influenza A(H5N1) viruses (Page 29)
Influenza A(H7N9)
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Real-time RT-PCR protocl for the detection of avian influenza A(H7N9) virus, provided by the WHO Collaborating Centre for Reference and Research in Beijing, China
pdf, 101kb
8 April 2013 (updated on 15 April)