False-negative RDT results and implications of new reports of P. falciparum histidine-rich protein 2/3 gene deletions
In 2010, a study sponsored by WHO and FIND found that some Plasmodium falciparum parasites in Peru lacked the pfhrp2 gene; without this gene, the parasite cannot produce HRP2 and cannot be detected by HRP2-based rapid diagnostic tests (RDTs). More recently, surveys in several African settings have also found a varying proportion of P. falciparum parasites lacking the pfhrp2 gene.
This updated information note provides countries with specific guidance on the implications of histidinerich protein 2/3 (pfhrp2/pfhrp3) gene deletions in P. falciparum parasites for case management and advises on procedures for investigating suspected false-negative RDT results. It supersedes the note published in May 2016.
- WHO investigates reports of missing gene among malaria parasites and possible impact on RDT performance
- Recommended selection criteria for procurement of malaria rapid diagnostic tests