False-negative RDT results and implications of new reports of P. falciparum histidine-rich protein 2/3 gene deletions


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Publication details

Number of pages: 11
Publication date: September 2017
Languages: English
WHO reference number: WHO/HTM/GMP/2017.18



In 2010, a study sponsored by WHO and FIND found that some Plasmodium falciparum parasites in Peru lacked the pfhrp2 gene; without this gene, the parasite cannot produce HRP2 and cannot be detected by HRP2-based rapid diagnostic tests (RDTs). More recently, surveys in several African settings have also found a varying proportion of P. falciparum parasites lacking the pfhrp2 gene.

This updated information note provides countries with specific guidance on the implications of histidinerich protein 2/3 (pfhrp2/pfhrp3) gene deletions in P. falciparum parasites for case management and advises on procedures for investigating suspected false-negative RDT results. It supersedes the note published in May 2016.

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