Preparation of Giemsa stock solution

Overview

The purpose of this document is to detail the procedure for preparing a stock solution of Giemsa stain for routine staining of malaria thick and thin blood films.

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Related publications

This document is part of a series of 18 SOPs on malaria microscopy:

• Cleaning and storing microscope slides (SOP 01)
• Preparation of Giemsa stock solution (SOP 02)
• Preparation of water buffered to pH 7.2 (SOP 03a)
• Preparation of water buffered to pH 7.2 with buffer tablets (SOP 03b)
• Quality control of Giemsa stock solution and buffered water (SOP 03c)
• Preparation of Giemsa working solution (SOP 04)
• Collection of finger-prick blood and preparation of thick and thin blood films (SOP 05a)
• Collection of blood by venipuncture and preparation of blood films from venous blood collected in tubes containing anticoagulant (SOP 05b)
• Labelling malaria blood films (SOP 06a)
• Recording and reporting microscopy results (SOP 06b)
• Giemsa staining of malaria blood films (SOP 07a)
• Ebola virus inactivation during staining of blood films with Giemsa stain (SOP 07b)
• Microscopy examination of thick and thin blood films for identification of malaria parasites (SOP 08)
• Malaria parasite counting (SOP 09)
• Preparation of blood spots on filter paper (SOP 10)
• General safety procedures in the malaria microscopy laboratory (SOP 11)
• Use, care and maintenance of microscopes (SOP 12)
• Management of wastes generated from malaria diagnostic tests (SOP 13)