Préparation de solution (‎eau)‎ tamponnée à pH 7,2 à partir de comprimés tampons

Vue d’ensemble

The objective of this document is to provide a detailed description of the procedure to be followed for the preparation of buffered solution (water) at pH 7.2 using buffer tablets for the preparation of a Giemsa working solution for use in routine staining of blood films for malaria.

Related publications

This document is one of a series of 18 standard operating procedures for the microscopic diagnosis of malaria:

• Cleaning and storage of microscopy slides ( SOP 01)
• Preparation of Giemsa stock solution ( SOP 02)
• Preparation of buffered solution at pH 7.2 ( SOP 03a)
• Preparation of pH 7.2 buffered solution (water) from buffer tablets ( SOP 03b)
• Quality control of Giemsa stock solution and buffered water ( SOP 04a)
• Preparation of a Giemsa working solution ( SOP 04b)
• Finger prick blood collection and preparation of thick drops and thin smears ( SOP 05a)
• Collecting blood samples by venipuncture and preparing blood filmsfrom venous blood collected in tubes containing an anticoagulant ( SOP 05b )
• Labeling of blood filmsfor plasmodium ( SOP 06a )
• Recording and notification of examination results ( SOP 06b)
• Giemsa staining of blood filmsfor plasmodium ( SOP 07a )
• Inactivation of Ebola virus when staining blood filmswith Giemsa ( SOP 07b )
• Microscopic examination of thin smears and thick drops for the identification of malaria parasites ( SOP 08)
• Parasite count( SOP 09 )
• Preparation of blood stains on filter paper ( SOP 10)
• General Safety Procedures in the Malaria Microscopic Diagnostic Laboratory ( SOP 11)
• Use, care and maintenance of microscopes ( SOP 12)
• Management of waste from malaria diagnostic tests ( SOP 13)